Part:BBa_K352001:Design

CooA from Rhodospirillum rubrum

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 448
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence information was acquired from NCBI and physical DNA was synthesized from GENEART. Classical cloning strategies(restriction digestion) were used to produce biobricks.

Source

Hwan Youn, Robert L. Kerby, Mary Conrad, et al. 2004. Functionally Critical Elements of CooA-Related CO Sensors. J. Bacteriol. 186(5):1320-1329. doi:10.1128/JB.186.5.1320-1329.2004.

References

- He Y, Shelver D, Kerby R.L., and Roberts G.P. (1996). Characterization of a CO-responsive transcriptional activator from Rhodospirillum rubrum. The Journal of Biological Chemistry 1996; 271(5):120-123.

- Shelver D, Kerby R.L., He Y, and Roberts G.P. (1997). CooA, a CO-sensing transcription factor from Rhodospirillum rubrum, is a CO-binding heme protein. Proceedings of the National Academy of Sciences 1997; 94: 11216-20.

- Aono S, Ohkubo K, Matsuo T, and Nakajima T (1998). Redox-controlled ligand exchange of the Heme in the CO-sensing transcriptional activator CooA. The Journal of Biologycal Chemistry 1998; 273(40): 25757-64.